Medical Laboratory Scientist (MLS) ASCP Exam 2025 – 400 Free Practice Questions to Pass the Exam

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What does the prozone effect typically result in during immunoassays?

False negative reactions

The prozone effect in immunoassays refers to a phenomenon where an excess of antibodies is present, leading to an insufficient number of antigens to form antigen-antibody complexes. This can prevent the proper formation of immune complexes, which are necessary for accurate assay results. When the prozone effect occurs, it generally results in false negative reactions, as the high concentration of antibodies can inhibit the visible reaction that would typically signal a positive result.

So while the answer provided indicates that there would be "no effect on results," this is not the case with the prozone effect—the presence of excess antibodies indeed affects the results by potentially masking the presence of antigens that are being tested for, leading to a misinterpretation of the test outcome.

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No effect on results

False positive reactions

Prolonged reaction times

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